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The foremost elements of the HPLC are revealed in Figure (PageIndex three ). The function of a pump is always to power a liquid (mobile phase) by way of at a particular stream price (milliliters for every moment). The injector serves to introduce the liquid sample into the flow stream of your mobile section. Column is considered the most central an
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The sample mixture for being separated and analyzed is introduced, within a discrete modest volume (usually microliters), in to the stream of cell stage percolating in the column. The factors of the sample go from the column, Each and every at a distinct velocity, which happen to be a functionality of unique physical interactions Along with the ads
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